Fig. 2.

In vitro effects of GSK-J4 on the motility and viability of S. mansoni adult worms and schistosomulum. a Adult worms were treated with different concentrations of GSK-J4, PZQ, GSK-J5 or DMSO alone and motility phenotypes were scored at different time points as described in Methods, each cell represents a couple of worms. b Kaplan-Meier survival curves of adult female and male schistosomes cultured for 4 days following treatment with different concentrations of GSK-J4 and controls. c Number of eggs released by couples of worms treated with GSK-J4 and controls, ***P < 0.001. Data is representative of one from three independent experiments. d Schistosomula (100 per well) were incubated in quadruplicate with serial dilutions of GSK-J4 or with vehicle (0.1% DMSO) for up to 5 days. The viability was expressed as percentage of the luminescence values relative to the control (DMSO). e Microscopic assessment of schistosomula treated with 6.25 μM GSK-J4 for 48 h revealing a granulation phenotype. Mean ± SD from three replicate experiments. Letters indicate significant differences between treatments (P < 0.05) (one-way ANOVA). Scale-bars: 100 µm