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. 2020 Feb 8;12(2):190. doi: 10.3390/v12020190

Figure 2.

Figure 2

Purification and characterization of in-vitro transcribed 5’ and 3’ terminal regions (TRs) of MVEV and PowV RNA. (A) Size exclusion chromatography (SEC) elution profile of purified RNAs where the x-axis represents elution volume and the y-axis represents absorbance at 260 nm; (B) Urea-PAGE demonstrating the purity of the individual pooled RNA fractions from SEC peaks (~13 to 14.5 mL). For comparison purposes, we have included 74 nts tRNA and 23S rRNA from Escherichia coli (C) Sedimentation coefficient distribution profiles of MVEV and PowV non-coding TRs obtained from sedimentation velocity-analytical ultracentrifugation (SV-AUC). The SV peaks at ~4.5 S for each RNA represent monomeric fractions. The sedimentation coefficient values are corrected to standard solvent conditions (20 °C in water).