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. 2020 Feb 8;12(2):190. doi: 10.3390/v12020190

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Characterization of MVEV and PowV terminal regions using small-angle X-ray scattering (SAXS). (A) A plot of scattering intensity (Log I(q)) versus scattering angle (q = 4πsinθ/λ) representing merged SAXS data for MVEV and PowV. (B) Guinier plots (plot of ln(I(q)) versus q²) representing the homogeneity of samples and allowing determination of R_g based on the low-angle region data. (C) Dimensionless Kratky plots (I(q)/I(0)*(q*R_g)² vs q*R_g) for all four RNA samples demonstrating their extended structures. (D) Pair-distance distribution (P(r)) plots for all four RNA samples representing their maximal particle dimensions and allowing the determination of R_g from the entire SAXS dataset.