Table 1.
Biophysical parameters of MVEV and PowV non-coding RNAs.
| Sample | MVEV 5’TR | MVEV 3’TR | PowV 5’TR | PowV 3’TR |
|---|---|---|---|---|
| Mw (kDa, sequence) | 31.38 | 31.57 | 36.92 | 37.80 |
| Sedimentation coefficient (S)∇ | 4.27 (4.08, 4.46) |
4.30 (4.20, 4.41) |
4.50 (4.42, 4.55) |
4.53 (4.52, 4.53) |
| I(0)# | 0.0098 ± 4.7 × 105 | 0.019 ± 1.4 × 105 | 0.019 ± 6.7 × 105 | 0.034 ± 8.2 × 105 |
| q.Rg range | 0.38 – 1.26 | 0.59 – 1.26 | 0.34 – 1.26 | 0.47 – 1.29 |
| Rg (Å)# | 29.01 ± 0.22 | 45.72 ± 0.48 | 34.65 ± 0.19 | 35.84 ± 0.13 |
| I(0)# ∆ | 0.098 ± 3.8 × 105 | 0.018 ± 8.1 × 105 | 0.019 ± 4.2 × 105 | 0.034 ± 8.9 × 105 |
| Rg (Å)∆ | 30.16 ± 0.11 | 46.16 ± 0.18 | 34.48 ± 0.06 | 38.39 ± 0.08 |
| Dmax (Å) ∆ | 100 | 150 | 105 | 130 |
| χ2* | ~0.78 | ~0.83 | ~0.78 | ~0.80 |
| NSD * | 0.71 ± 0.01 | 0.80 ± 0.02 | 0.85 ± 0.02 | 0.72 ± 0.01 |
The Mw values were calculated using nucleotide sequences. ∇- determined using SV-AUC analysis and UltraScan-III package [60]. Sedimentation coefficients obtained following genetic algorithm-Monte Carlo analysis. The measured value represents the mean. Bracketed values represent a 95% confidence interval from Monte Carlo analysis. # - obtained from Guinier analysis [44]. ∆ - determined using P(r) analysis using the GNOM program [47]. * - values derived from DAMMIN [48] and DAMAVER [50] analysis.