Figure 2.

Depletion of TRIM41 increases host susceptibility to VSV infection. (A) A549 cells were transfected with 5 pmol of the control siRNA or the indicated siRNA duplex against TRIM41. After 48 h, the cells were infected at an MOI of 0.1 with VSV-Luc for 12 h. Relative VSV activities were determined by the luciferase activities that were normalized to the control. All experiments were biologically repeated three times. Data represent means ± standard deviations of three independent experiments. The p value was calculated (two-tailed Student’s t-test) by comparison of results with those of the siRNA control in each cell group. An asterisk indicates p < 0.05. (B) Wild type (WT) and TRIM41 knockout (KO) HEK293 cells were infected with the indicated MOIs of VSV-Luc for 12 h. Relative VSV activities were determined by the luciferase activities that were normalized to the control. All experiments were biologically repeated three times. The p value was calculated (two-tailed Student’s t-test) by comparison with the wild type. An asterisk indicates p < 0.05. (C) Wild type and TRIM41 knockout cells were infected with 0.001 MOI of VSV. After the designated hour post-infection, virus titers were determined by TCID₅₀ in Vero cells. All experiments were biologically repeated three times. The p value was calculated (two-tailed Student’s t-test) by comparison with the wild type. An asterisk indicates p < 0.05.