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. 2020 Jan 22;12(2):131. doi: 10.3390/v12020131

Figure 3.

Figure 3

TRIM41 co-localizes and interacts with the N protein of VSV. (A) HA-tagged TRIM41 (TRIM41-HA) was co-transfected with FLAG-tagged phosphoprotein (P), glycoprotein (G), or nucleoprotein (N) of VSV into HEK293 cells. Cells were treated with 1 µM proteasome inhibitor MG132 to prevent VSV protein from degradation. Cell lysates were immunoprecipitated with the anti-FLAG antibody and then blotted as indicated. (B) TRIM41-HA was co-transfected with FLAG-tagged matrix protein (M) or different doses of nucleoprotein (N) of VSV into HEK293 cells. Cells were treated with 1 µM proteasome inhibitor MG132 to prevent VSV protein from degradation. Cell lysates were immunoprecipitated with the anti-FLAG antibody and then blotted as indicated. (C) A549 cells were transfected with TRIM41-HA and FLAG-tagged VSV-N (VSV-N-FLAG). Cells were treated with 1 µM MG132 to prevent VSV protein from degradation. Red: FLAG; Green: HA; Blue: DAPI, a nuclear stain. (D) A549 cells were mock-infected or infected with VSV. Cells were treated with 1 µM MG132 to prevent VSV-N from degradation. After 24 h, cells were fixed with cold methanol and incubated with anti-VSV-N and anti-TRIM41 antibodies. Red: VSV-N; Green: TRIM41; Blue: DAPI, a nuclear stain.