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. 2020 Feb 8;12(2):187. doi: 10.3390/v12020187

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Immunoblot analysis of pIRF3 in porcine AMs. (a) Immunoblot analysis of pIRF3 in mock cells, poly (I:C)-stimulated cells, PRRSV-infected cells, PRRSV+PNI-infected cells or PRRSV+ICs-infected cells. (b) Immunoblot analysis of pIRF3 in cells treated with RNI, anti-FcγRI IgG or anti-FcγRIII IgG. (c) Immunoblot analysis of pIRF3 in poly (I:C)-stimulated cells pretreated with RNI, anti-FcγRI IgG or anti-FcγRIII IgG. (d) Immunoblot analysis of pIRF3 in PRRSV-infected cells pretreated with RNI, anti-FcγRI IgG or anti-FcγRIII IgG. The GAPDH served as a loading control. Note: PRRSV+PNI: PRRSV+porcine-negative IgG; PRRSV+ICs: PRRSV+porcine IgG against PRRSV; RNI: rabbit-negative IgG; pIRF3: phosphorylated interferon regulatory factor 3; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; FcγRI: Fc gamma receptor I; FcγRIII: Fc gamma receptor III.

Immunoblot analysis of pIRF3 in porcine AMs. (a) Immunoblot analysis of pIRF3 in mock cells, poly (I:C)-stimulated cells, PRRSV-infected cells, PRRSV+PNI-infected cells or PRRSV+ICs-infected cells. (b) Immunoblot analysis of pIRF3 in cells treated with RNI, anti-FcγRI IgG or anti-FcγRIII IgG. (c) Immunoblot analysis of pIRF3 in poly (I:C)-stimulated cells pretreated with RNI, anti-FcγRI IgG or anti-FcγRIII IgG. (d) Immunoblot analysis of pIRF3 in PRRSV-infected cells pretreated with RNI, anti-FcγRI IgG or anti-FcγRIII IgG. The GAPDH served as a loading control. Note: PRRSV+PNI: PRRSV+porcine-negative IgG; PRRSV+ICs: PRRSV+porcine IgG against PRRSV; RNI: rabbit-negative IgG; pIRF3: phosphorylated interferon regulatory factor 3; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; FcγRI: Fc gamma receptor I; FcγRIII: Fc gamma receptor III.