Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Feb 8;12(2):187. doi: 10.3390/v12020187

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Flow cytometry analysis of FcγRI or FcγRIII on the surface of porcine AMs. The cells were transfected with FcγRI siRNA, FcγRIII siRNA or negative siRNA for 48 h. (a) Representative histograms of the percentage of negative siRNA-transfected cells surface-stained with anti-FcγRI IgG and FITC-conjugated goat anti-rabbit IgG. (b) Representative histograms of the percentage of FcγRI siRNA-transfected cells surface-stained with anti-FcγRI IgG and FITC-conjugated goat anti-rabbit IgG. (c) Representative histograms of the percentage of negative siRNA-transfected cells surface-stained with anti-FcγRIII IgG and FITC-conjugated goat anti-rabbit IgG. (d) Representative histograms of the percentage of FcγRIII siRNA-transfected cells surface-stained with anti-FcγRIII IgG and FITC-conjugated goat anti-rabbit IgG. (e) Representative histograms of the percentage of mock cells surface-stained with RNI and FITC-conjugated goat anti-rabbit IgG. Note: siRNA: small interfering RNA; FcγRI: Fc gamma receptor I; FcγRIII: Fc gamma receptor III; FITC: fluorescein isothiocyanate; RNI: rabbit-negative IgG.

Flow cytometry analysis of FcγRI or FcγRIII on the surface of porcine AMs. The cells were transfected with FcγRI siRNA, FcγRIII siRNA or negative siRNA for 48 h. (a) Representative histograms of the percentage of negative siRNA-transfected cells surface-stained with anti-FcγRI IgG and FITC-conjugated goat anti-rabbit IgG. (b) Representative histograms of the percentage of FcγRI siRNA-transfected cells surface-stained with anti-FcγRI IgG and FITC-conjugated goat anti-rabbit IgG. (c) Representative histograms of the percentage of negative siRNA-transfected cells surface-stained with anti-FcγRIII IgG and FITC-conjugated goat anti-rabbit IgG. (d) Representative histograms of the percentage of FcγRIII siRNA-transfected cells surface-stained with anti-FcγRIII IgG and FITC-conjugated goat anti-rabbit IgG. (e) Representative histograms of the percentage of mock cells surface-stained with RNI and FITC-conjugated goat anti-rabbit IgG. Note: siRNA: small interfering RNA; FcγRI: Fc gamma receptor I; FcγRIII: Fc gamma receptor III; FITC: fluorescein isothiocyanate; RNI: rabbit-negative IgG.