Table A2.
The amino acid multiple reaction monitoring (MRM) transitions quantifier ion (first transition listed) and qualifier ion (second transition listed), chromatographic retention times, cone voltages, and collision energies.
| Amino Acid | Mass AA, g/mol | MRM Transition | Retention Time, min | Cone Voltage, V | Collision Energy, eV |
|---|---|---|---|---|---|
| Ala | 89.1 | 90.1 > 44.0 | 6.50 | 15 | 9 |
| Arg | 174.2 | 175.1 > 70.1 | 11.06 | 25 | 20 |
| 175.1 > 116.0 | 11.06 | 25 | 19 | ||
| Asn | 132.1 | 133.0 > 74.0 | 9.05 | 20 | 15 |
| 133.0 > 88.0 | 9.05 | 20 | 10 | ||
| Asp | 133.1 | 134.0 > 74.0 | 10.57 | 20 | 14 |
| 134.0 > 88.0 | 10.57 | 20 | 14 | ||
| Gln | 146.2 | 147.0 > 84.0 | 8.80 | 15 | 15 |
| 147.0 > 102.0 | 8.82 | 15 | 19 | ||
| Glu | 147.1 | 148.0 > 84.0 | 9.64 | 18 | 16 |
| 148.0 > 102.0 | 9.63 | 18 | 15 | ||
| Gly | 75.1 | 76.0 > 30.0 | 8.00 | 20 | 7 |
| 76.0 > 48.0 | 8.02 | 20 | 7 | ||
| Ile | 131.2 | 132.0 > 69.0 | 2.99 | 15 | 15 |
| 132.0 > 86.0 | 2.97 | 15 | 15 | ||
| Leu | 131.2 | 132.0 > 30.0 | 2.69 | 15 | 15 |
| 132.0 > 86.0 | 2.70 | 15 | 15 | ||
| Lys | 146.2 | 147.0 > 84.0 | 11.42 | 20 | 15 |
| 147.0 > 130.0 | 11.42 | 20 | 13 | ||
| Met | 149.2 | 150.0 > 104.0 | 3.38 | 16 | 10 |
| 150.0 > 133.0 | 3.36 | 16 | 10 | ||
| Phe | 165.2 | 166.0 > 120.0 | 2.62 | 20 | 15 |
| 166.0 > 103.0 | 2.62 | 20 | 20 | ||
| Pro | 115.1 | 116.0 > 70.1 | 4.04 | 15 | 15 |
| 116.0 > 43.1 | 4.02 | 15 | 15 | ||
| Ser | 105.1 | 106.0 > 60.0 | 8.83 | 20 | 9 |
| 106.0 > 42.0 | 8.85 | 20 | 15 | ||
| Thr | 119.1 | 120.0 > 74.0 | 7.55 | 15 | 11 |
| 120.0 > 56.0 | 7.51 | 15 | 11 | ||
| Trp | 204.2 | 205.0 > 146.0 | 2.66 | 15 | 18 |
| 205.0 > 188.0 | 2.68 | 15 | 18 | ||
| Tyr | 181.2 | 182.0 > 136.0 | 4.24 | 20 | 15 |
| 182.0 > 165.0 | 4.23 | 20 | 19 | ||
| Val | 117.1 | 118.0 > 72.0 | 3.90 | 18 | 10 |
| 118.0 > 55.0 | 3.90 | 18 | 10 |