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. 2020 Feb 20;12(2):236. doi: 10.3390/v12020236

Table 1.

Primary cell cultures from hymenopteran species.

Species Tissue Longevity Medium Incubation Year Ref
Pseudomyrmex triplarinus Venom glands 1 year PTM-1CC 28 °C 1985 [26]
Apis mellifera Antennal lobes ~1 month 5+4 and A2 29 °C 1991 [30]
Apis mellifera Pupal honey bee brain Three weeks L-15 29 °C 1992 [31]
Mormoniella vitripennis Eggs 3 months Grace 28 °C 1993 [29]
Apis mellifera Mushroom body NA L-15 NA 1994 [32]
Apis mellifera Kenyon cells Up to 10 days L-15 29°C 1994 [33]
Apis mellifera Antennal lobe NA 5+4 NA 1994 [34]
Apis mellifera Antennal flagella Several weeks 5+4 30 °C 1994 [35]
Apis mellifera Kenyon cells Up to 6 weeks L-15 26 °C 1999 [36]
Apis mellifera Antennal motor neurons NA L-15 28 °C 1999 [37]
Apis mellifera Kenyon cells and projection neurons NA L-15 26 °C 2003 [38]
Apis mellifera Mushroom bodies neuroblasts NA L-15 26 °C 2003 [39]
Apis mellifera Antennal lobes ~1 month L-15 26 °C 2008 [40]
Apis mellifera Pre-gastrulastage
embryos
More than 3 months Grace 30 °C 2006 [44]
Apis mellifera Eggs Four months Grace’s or Schneider’s 32 °C with 5% CO2 2010 [45]
Apis mellifera Pupae At least 8 days WH2 22 °C 2010 [47]
Apis mellifera Gut At least 6 days L-15 33 °C 2012 [46]
Apis mellifera Midgut 15 days WH2 27 °C 2012 [41]
Apis mellifera Eggs ~135 day L-15 30 °C 2015 [43]