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. 2020 Feb 23;12(2):249. doi: 10.3390/v12020249

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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The third domain of cheEF1α interacted with vvIBDV VP3. (A) The expression plasmids harboring different domains of cheEF1α were depicted as cheEF1α-DI, cheEF1α-DII, cheEF1α-DIII, cheEF1α-DI+II, and cheEF1α-DII+III. (B) 293T cells were transfected expression plasmids with HA-vvIBDV VP3 and Flag-cheEF1α, HA-vvIBDV VP3 and Flag-cheEF1α-DI, HA-vvIBDV VP3 and Flag-cheEF1α-DII, HA-vvIBDV VP3 and Flag-cheEF1α-DIII, HA-vvIBDV VP3 and Flag-cheEF1α-DI+II, or HA-vvIBDV VP3 and Flag-cheEF1α-DII+III. Cell lysates were prepared at 36 h post transfection and immunoprecipitated with anti-Flag antibody and immunoblotted with anti-Flag or anti-HA antibodies. Each co-immunoprecipitation experiment was repeated three times.