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. 2020 Jan 31;12(2):162. doi: 10.3390/v12020162

Figure 1.

Figure 1

E2-2 expression in plasmacytoid dendritic cells (pDCs). (A) Gating strategy to identify pDCs from human peripheral blood mononuclear cells ( PBMCs). (B) Gating to determine removal of AXL+ Siglec 6+ DCs after negative selection for pDCs. (C) E2-2 expression in CD3+ and CD11c+ cells compared to pDCs as determine by flow cytometry. Representative histogram on the left, quantified mean fluorescent intensity (MFI) on the right. n = 11 independent experiments. (D) PBMCs were stimulated with 10 µM R848 for up to 6 h and E2-2 expression was measured via flow cytometry. n = 8 independent experiments. (E) mRNA expression from PBMCs measured by qRT-PCR after 6 h R848 stimulation. n = 3 independent experiments. Data are presented as means ± SEM. p values < 0.05 were considered significant. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.