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. Author manuscript; available in PMC: 2020 Aug 7.
Published in final edited form as: Chem Commun (Camb). 2019 Jul 10;55(61):8959–8962. doi: 10.1039/c8cc10192b

Figure 2.

Figure 2.

A mock selection cycle using stapled mRNA display to measure enrichment of E2 binding peptide 10. P = puromycin. mRNAs corresponding to peptide 10 and the library mRNAs were each ligated to a puromycin DNA sequence. These puromycin-mRNAs were then added to in vitro translation reactions, leading to fusion of the nascent linear peptides onto their encoding mRNAs. Each mRNA-peptide fusion was then cyclized with DBX, followed by reverse transcription to generate the cDNA. The mRNA-peptide fusions were then captured onto Avi-tagged HPV16 E2.44 The non-binding mRNA-peptide fusions were washed away, and binders were then eluted with heat and amplified by PCR.