Skip to main content
. Author manuscript; available in PMC: 2020 Aug 7.
Published in final edited form as: Chem Commun (Camb). 2019 Jul 10;55(61):8959–8962. doi: 10.1039/c8cc10192b

Figure 3. In vitro selection showing enrichment of mRNA corresponding to peptide 10.

Figure 3.

(A) Scheme illustrating how a ClaI site can be used to distinguish between DNAs encoding the naïve library (lib) or our E2-binding peptide derived from 10 (E2) (B) Agarose EtBr stained gel showing DNAs corresponding to the library (Lib) and the E2 binder (E2) alone, as well as a mixture of both before (Pre) and after (Post) one round of in vitro selection (right). The ratio of Library to E2 was calculated using ImageJ by taking the top band (Lib) and dividing it by the sum of the bottom two bands (E2).