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. 2020 Mar 10;11:353. doi: 10.3389/fimmu.2020.00353

FIGURE 5.

FIGURE 5

LXR priming increases Acetyl-CoA levels, a novel mediator of inflammatory priming. (A–C) Monocytes were treated as indicated with 2 μM T1317 (LXR agonist), 5 μM GSK2033 (LXR antagonist), 500 μM Acetyl-CoA, 25 μM C75 (FASN-inhibitor) or vehicle for 24 h. (A) cells were lysed and acetyl-CoA concentration measured on day 3. (B,C) cells were kept for 5 days in complete medium and restimulated with 5 μg/ml Pam3cys for 24 h. IL-6 was measured in the supernatant. (D) monocytes were transfected with siRNA against SREBP1 or scrambled siRNA, treated with 2 μM T1317 or vehicle for 24 h and kept for 5 days in complete medium. Then cells were restimulated with 5 μg/ml Pam3cys for 24 h and IL-6 was measured in the supernatant. Graphs represent mean values ± SD of six individuals in three different experiments. *P < 0.05, **P < 0.01, and ***P < 0.001.