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. 2020 Mar 10;11:353. doi: 10.3389/fimmu.2020.00353

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Copyright © 2020 Sohrabi, Sonntag, Braun, Lagache, Liebmann, Klotz, Godfrey, Kahles, Waltenberger and Findeisen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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LXR priming is dependent on IL-1β. Monocytes were treated as indicated with 2 μM T1317 (LXR agonist), 10 μM GW3965 (LXR agonist), 5 μM GSK2033 (LXR antagonist), 1 μM Diacerein, 10 ng/ml IL-1β, 200 ng/ml IL1RA (IL-1 receptor antagonist) or vehicle for 24 h. (A) mRNA levels of IL-1β, were analyzed by real-time qPCR. (B) 24 h after priming medium was changed and after 24 h resting time supernatant was collected and the level of IL-1β was measured using ELISA. (C–F) cells were kept for 5 days in complete medium and restimulated with 5 μg/ml Pam3cys for 24 h. IL-6 (C–E) and TNFα (F) were measured in the supernatant. Graphs represent mean values ± SD of six individuals in three different experiments. *P < 0.05, **P < 0.01 and ***P < 0.001.