Skip to main content
. 2020 Feb 5;12(2):329. doi: 10.3390/polym12020329

Figure 5.

Figure 5

Effect of freezing conditions (standard method (STD), NFD, and IFD at −20 and −210 °C) over human dermal fibroblasts’ (hDFbs) behavior in 1.25% GG spongy-like hydrogels. (A) Representation of the entrapment efficiency 24 h after cell seeding. (B) (i) Representation of the percentage of the live cells three and seven days of culture. (ii) Representative fluorescence microscopy images showing the dead (red) and the live (green) cells, respectively stained with propidium iodide (PI) and calcein-AM (Ca-AM) after three and seven days of culture. Scale bar = 200 µm. (C) Representative fluorescence microscopy images of hDFbs after three and seven days of culture showing the F-actin cytoskeleton (phalloidin-TRITC, red) and nuclei (DAPI, blue). Scale bar = 50 µm. Data were obtained from three independent experiments with three replicates for each condition, * p < 0.05, two-way ANOVA and Bonferroni’s post-hoc test.