FIGURE 3.

Pseudomonas aeruginosa SODB targets the phagocytic ${\text{O}}_2^{\text{-}}$. Measurements of superoxide anions using luminol in RAW264.7 macrophages (A,B) and HMDMs (C,D). Representative time courses of luminol chemiluminescence in the untreated cells (A,C, left panel) and cells pretreated with the NADPH oxidase inhibitor diphenyleneiodonium (A,C, right panel) (DPI; 10 μM), and infected with the indicated P. aeruginosa strains at a multiplicity of infection of 10. Data are means ± standard deviation of three technical replicates. (B,D) Quantification of ${\text{O}}_2^{\text{-}}$, as determined by the area under the curve (AUC) from three independent experiments, each carried out in triplicate. RLU, relative luminescent units; * P < 0.05 (Student’s t-tests).