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. 2020 Mar 10;11:326. doi: 10.3389/fmicb.2020.00326

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Copyright © 2020 Cavinato, Genise, Luly, Di Domenico, Del Porto and Ascenzioni.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Bacterial SODB enhances long-term intracellular survival within macrophages. Survival curves of Pseudomonas aeruginosa in RAW264.7 macrophages (A,B) and HMDMs (D,E). The cells were infected with the indicated P. aeruginosa strains, and untreated and pretreated with the NADPH oxidase inhibitor diphenyleneiodonium (DPI; 10 μM for 30 min), as indicated. At the end of the infection macrophages (time 0) and after 60 and 180 min, samples were collected and the CFU determined. (C,F) Bacterial survival in untreated RAW264.7 (C) and HMDMs (F) at 180 min from the end of infection. Data are means ± standard deviation of three independent experiments. * P < 0.05; ** P < 0.01 (Student’s t-tests).