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. 2020 Mar 10;11:326. doi: 10.3389/fmicb.2020.00326

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Copyright © 2020 Cavinato, Genise, Luly, Di Domenico, Del Porto and Ascenzioni.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Pseudomonas aeruginosa SODB reduces autophagy activation in macrophages. RAW264.7 macrophages (A,B) and HMDMs (C,D) were infected with the indicated P. aeruginosa strains. After infection, whole cell lysates were immunoblotted with anti-LC3 and anti-GAPDH (loading control) antibodies. (A,C) Representative Western blots. (B,D) Quantitative analysis of band intensities, reported as the LC3-II/GAPDH ratio. Data are means ± standard deviation of three independent experiments. Ctr, uninfected macrophages. * P < 0.05; *** P < 0.001 (Student’s t-tests).