Table 1.
Characterization of thermoresponsive substrates for cell culture.
| Substrate Type | Measurement Data | Detection Techniques |
|---|---|---|
| PNIPAAm homopolymers and copolymers | Chemical composition | Element analysis, FTIR, NMR |
| Molecular weight | SEC, SLS, MS | |
| Phase transition temperature Lower critical solution temperature | DSC, DLS, turbidity measurement | |
| Thermoresponsive planar films | Chemical composition | XPS, SFG, TOF-SIMS |
| Grafting density | Gravimetric method, ATR-FTIR | |
| Surface topology | SEM, AFM, profilometer | |
| Surface wettability | Contact angle measurement | |
| Film thickness | SE, SPR | |
| Thermoresponsive hydrogels | Chemical composition | FTIR, NMR, XPS |
| Thermoresponsive swelling | DLS, DSC, ATR-FTIR, dynamic rheology, gravimetric method | |
| Mechanical properties | Shear-flow treatment, compression assay, mechanical stress | |
| Architecture | Raman microscopy, SEM | |
| Thermoresponsive carriers | Chemical composition | Elemental analysis, ATR-FTIR, NMR, XPS, SEM/EDS system |
| Size and morphology | Optical microscopy, SEM, AFM | |
| Thermoresponsive swelling | Dynamic rheology, DLS, optical microscopy | |
| Thermoresponsive scaffolds | Chemical composition | ATR-FTIR, NMR, XPS, SEM/EDS system |
| Morphology and structure | SEM, AFM, optical microscopy, digital calipers, liquid displacement method, BET, BJH | |
| Surface wettability | Contact angle measurement | |
| Thermoresponsive swelling | Gravimetric method | |
| Mechanical properties | Compression assay, mechanical stress |