Figure 2.

Quantitative RT-PCR (qRT-PCR). (A) Box and Whisker plot with jitter showing qRT-PCR of WT-GHR mRNA fold change relative to control. Box plots show the mean, upper and lower quartiles and range; IQR = interquartile range; P values calculated by one way ANOVA with Bonferroni correction. *** P value <0.001. (B) Box and Whisker plot with jitter showing qRT-PCR of 6Ψ-GHR mRNA fold change relative to Pt 1. Box plots show the mean, upper and lower quartiles and range; IQR = interquartile range; P values calculated by one-way ANOVA with Bonferroni correction. ***P value <0.001; **P value = 0.017. (C) Scatter plot showing the correlation between the height SDS at presentation and the mean 6Ψ:WT transcript ratios in the four 6Ψ patients. Pt, patient; 6Ψ, pseudoexon; WT, wild type; R, Pearson correlation coefficient.