Figure 3.

miR‐1254 promoted the apoptosis and blocked the cell cycle of glioma cells U87 and U251. (A) Results of flow cytometry showed that cell apoptosis rate was notably higher in miR‐1254 overexpression group while lower in anti‐miR‐1254 group. (B) Cell cycle progression of cells in miR‐1254 group was arrested in G0/G1 phase. (C) Western blotting of cyclin D1 and CDK4 in U87 and U251 cells after transfection with the indicated constructs. β‐Actin was used as the loading control. Results were expressed as means ± SD of 3 independent experiments. *P < .05; **P < .01