Figure 4.

The effect of quercetin on wear particle‐mediated macrophage polarization was further verified in vitro. We confirmed by immunofluorescence (A) that quercetin can inhibited Ti particle‐mediated M1 macrophage polarization and promoted M2 macrophages. We also verified the polarization of this macrophage by flow cytometry (B). The results of flow cytometry were also statistically analysed (C, D). The number of cells collected by flow cytometry is known. If Ti promotes the polarization of M1 macrophages, it is bound to weaken the number of M2 macrophages. Therefore, the number of the CD206 in Figure 5D was significantly reduced