Figure 5.

Expression of smooth muscle α‐actin and F‐actin. Western analysis of (A) smooth muscle α‐actin (ASMA) and (B) F‐actin expression in aortas from Ins2^Akita, ApoE^−/− and Ins2^Akita: ApoE^−/− mice and from sex‐ and age‐matched (3 months old) C57BL/6 non‐diabetic control mice, with GAPDH serving as a loading control. The bar graph represents for each value the mean ± SD from three separate experiments. *P < .05 vs C57BL/6 control mice; §P < .05 vs Ins2^Akita mice and ApoE^−/− mice; N = 8 mice/group