Figure 3.

Expression of OPN mRNA in NHF, HUVSMC and HUVEC is not induced in hypoxia. (A, C and E) Histograms represent the expression of the mRNA of GLUT‐1 in NHF (A), HUVSMC (C) and HUVEC (E). The mean ± SEM is representative of three independent experiments. Cells were cultured in the absence (Ctl) or presence of H₂O₂ (50 µmol/L), or glucose (50 mmol/L) for 48 h, or transfected with control siRNA (siCtl) and glucose (Gluc) + siOPN (40 nmol/L) in normoxia. Cells were cultured in the absence (Ctl) or presence of an inhibitor to (iNF‐kB) (10 µmol/L) for 48 h or transfected with control siRNA (siCtl), siHIF‐1α (40 nmol/L), siHIF‐2α (40 nmol/L), siHIF‐1/2α (40 nmol/L + 40 nmol/L) or siOPN (40 nmol/L), and incubated in hypoxia 1% O₂ (Hx) for 48 h. (B, D and F) Histograms represent the expression of the mRNA of OPN in NHF (B), HUVSMC (D) and HUVEC (F). The mean ± SEM is representative of three independent experiments. Cells were cultured in the absence (Ctl) or presence of H₂O₂ (50 µmol/L), glucose (50 mmol/L) for 48 h, or transfected with control siRNA (siCtl) and glucose (Gluc) + siOPN (40 nmol/L) in normoxia. Cells were cultured in the absence (Ctl) or presence of iNF‐kB (10 µmol/L) for 48 h or transfected with control siRNA (siCtl), siHIF‐1α (40 nmol/L), siHIF‐2α (40 nmol/L), siHIF‐1/2α (40 nmol/L + 40 nmol/L) or siOPN (40 nmol/L), and incubated in hypoxia 1% O₂ (Hx) for 48 h. HUVEC, human umbilical vein endothelial cells; HUVSMC, Human umbilical vein smooth muscle cells; NHF, normal human fibroblasts; OPN, osteopontin