Table 1.
Primer sequences for RT‐qPCR
| Genes | Forward sequences (5′‐3′) | Reserve primers (5′‐3′) |
|---|---|---|
| miR‐192 | CTCGAGGACTGGGGAGCCCAGGTGAG | GGATCCGAGGCAGCAAATGCTTGGACAG |
| miR‐96 | GCCCGCTTTGGCACTAGCACATT | GTGCAGGGTCCGAGGT |
| let‐7g‐5p | GAAGCAGCCAGCCCAAAGT | GGAGGAGCAGAACTGAACCC |
| U6 | TTCAACTATGGCAGCTCGGT | AACAACAGAGGCCAGCGTTA |
| HMGA2 | CACAAGAGTCTGCCGAAGAGG | GAAGAACAACGGGCTGGTCG |
| β‐Catenin | CTGAGATCCCCCTGCTTTCC | ATAGCCAGGGTTAGCTCAGTG |
| LC3 | GCGAGTTACCTCCCGCAG | TCATGTTGACATGGTCCGGG |
| Beclin1 | CTCCCGAGGTGAAGAGCATC | GCTGTTGGCACTTTCTGTGG |
| Bax | GCTGCCTTGGACTGTGTTTTT | CAGATGCCGAAGTGTGTCCC |
| Bcl‐2 | GAACTGGGGGAGGATTGTGG | CATCCCAGCCTCCGTTATCC |
| Caspase‐3 | GTCCTGGGACACCGGTTAT | ACGGCAGGCCTGAATAATGA |
| PKC | CTGGTCATCGCTAACATAG | GTTAAGAATCACTTCCCACT |
| GAPDH | AATGGGCAGCCGTTAGGAAA | GCGCCCAATACGACCAAATC |
All genes above were all obtained from NCBI.
Abbreviations: Bax, Bcl‐2 associated X protein; Bcl‐2, B‐cell lymphoma‐2; Caspase, cysteinyl aspartate specific proteinase; GAPDH, glyceraldehyde‐3‐phosphate dehydrogenase; HMGA2, high mobility group A2; LC3, Autophagy marker Light Chain 3; let‐7g‐5p, microRNA let‐7g‐5p; NCBI, National Center for Biotechnology Information; PKC, protein kinase C; RT‐qPCR, reverse transcription quantitative polymerase chain reaction; U6, U6 small nuclear.