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. 2020 Jan 22;24(5):3139–3148. doi: 10.1111/jcmm.14983

Figure 2.

Figure 2

VitB6 inhibits LPS‐induced inflammation in cultured macrophages. Cultured macrophages were pretreated with vitamin B6 in different concentrations for two hours and then incubated with LPS (100 ng/mL) for 24 h. (A‐C) The protein levels of IL‐1β in A, TNF‐α in B and IL‐6 in C in culture medium were quantified by ELISA. (D‐F) The mRNA levels of IL‐1β in D, TNF‐α in E and IL‐6 in F were assayed by real‐time PCR. N = 3‐7 per group. *P < .05 vs Control. # P < .05 vs LPS