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. 2020 Feb 6;24(5):2772–2790. doi: 10.1111/jcmm.15030

Figure 4.

Figure 4

miR24‐2 increases the miR6079 expression via RNA methylation modification. A, Western blotting with anti‐METTL3 in Hep3B infected with rLV, rLV‐miR24‐2, respectively. B, RNA Immunoprecipitation (RIP) with anti‐METTL3 followed by RT‐PCR with pre‐miR6079 primers in Hep3B infected with rLV, rLV‐miR24‐2, respectively. Western blotting with anti‐METTL3 for each sample. C, a, RNA immunoprecipitation (RIP) with anti‐M6A followed by RT‐PCR with pre‐miR6079 primers. b, RNA immunoprecipitation (RIP) with anti‐M6A followed by real‐time RT‐PCR. D, Northern blotting analysis of miR6079 in Hep3B infected with rLV, rLV‐miR24‐2, rLV‐miR24‐2 plus pGFP‐V‐RS‐METTL3 and rLV‐miR24‐2 plus Cycloleucine, respectively. U6 as internal control. Western blotting with anti‐METTL3 for each sample and β‐actin as internal control. E, The real‐time RT‐PCR analysis. U6 as internal control. Each value was presented as mean ± standard error of the mean (SEM) (Student's t test). Bar ± SEM. **P < .01; *P < .05