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. 2020 Feb 6;24(5):2772–2790. doi: 10.1111/jcmm.15030

Figure 5.

Figure 5

miR6079 enhances H3K9me3 by targeting for JMJD2A. A, a, the green protein is observed under a fluorescence microscope in the two stable Hep3B cell lines by infecting with rLV or rLV–miR6079, respectively (original magnification ×100, scale bars, 400 μm). B, The Northern‐Western blot analysis for miR‐6079 in the two stable Hep3B cell lines by infecting with rLV or rLV‐miR24‐2 respectively. U6 as internal control. c, The RT‐PCR analysis for pre‐miR6079. d, The real‐time RT‐PCR analysis for mature miR6079. Each value was presented as mean ± standard error of the mean (SEM) (Student's t test). Bar ± SEM. **P < .01; *P < .05. B, The informatics analysis of miR6079 targeting for JMJD2A 3′UTR using MirTarget scanning soft. C, The assay of JMJD2A 3′‐UTR luciferase activity. D, The RT‐PCR analysis for JMJD2A. β‐Actin as internal control. E, a, Western blotting with anti‐JMJD2A. b, Grey density analysis of band. F, a, Co‐immunoprecipitation (IP) with anti‐JMJD2A followed by Western blotting with anti‐histone H3. Western blotting with anti‐JMJD2A for each sample. b, Grey density analysis of band. G, Western blotting with anti‐H3K9me3 and anti‐JMJD2A. β‐Actin as internal control