Skip to main content
. 2020 Feb 6;24(5):2772–2790. doi: 10.1111/jcmm.15030

Figure 6.

Figure 6

miR24‐2 enhances H3K9me3 by inhibiting JMJD2A A, The assay of JMJD2A 3′‐UTR luciferase activity. Each value was presented as mean ± standard error of the mean (SEM).**P < .01. B, a, Western blotting with anti‐JMJD2A. b, Grey density analysis of band. C, a, Co‐immunoprecipitation (IP) with anti‐JMJD2A followed by Western blotting with anti‐histone H3. Western blotting with anti‐JMJD2A for each sample. b, Grey density analysis of band. D. Western blotting with anti‐H3K9me3 and anti‐JMJD2A. β‐Actin as internal control. E, a. The RT‐PCR analysis for β‐actin. b. The RT‐PCR analysis for pre‐miR24‐2. c. The real‐time RT‐PCR analysis for mature miR24‐2. U6 as internal control. d. The real‐time RT‐PCR analysis for mature miR6079