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. 2020 Feb 6;24(5):2772–2790. doi: 10.1111/jcmm.15030

Figure 8.

Figure 8

miR24‐2 enhances Pim1 through H3K9me3 and METTL3. A, a, Chromatin immunoprecipitation (CHIP) with anti‐H3K9me3 followed by PCR with Pim1 promoter primers. b. Real‐time CHIP analysis. Each value was presented as mean ± standard error of the mean (SEM).**P < .01. B, Chromatin immunoprecipitation (CHIP) with anti‐RNA pol II followed by PCR with Pim1 promoter primers. IgG CHIP as negative control. Pim1 promoter as INPUT. Western blotting with anti‐RNA pol II for each sample. C, The assay of Pim1 promoter luciferase activity. D, RNA immunoprecipitation (RIP) with anti‐METTL3 followed by RT‐PCR with Pim1 3′UTR primers. Western blotting with anti‐METTL3 for each sample. E, RNA immunoprecipitation (RIP) with anti‐M6A followed by RT‐PCR with Pim1 3′UTR primers. F, The assay of Pim1 3′UTR luciferase activity. G, The RT‐PCR for Pim1. β‐Actin as internal control. H, Western blotting with anti‐Pim1. β‐Actin as internal control. I, The RT‐PCR for Pim1. J, Western blotting with anti‐Pim1. β‐Actin as internal control