Figure 10.

The rescued test for Pim1 in three stable Hep3B cell lines, including rLV group, rLV‐miR24‐2 group and rLV‐miR24‐2 plus pRFP‐C‐RS‐Pim1 group, respectively. A, a, The Western blotting analysis with anti‐Pim1. β‐Actin as internal control. B, The real‐time RT‐PCR analysis for mature miR24‐2. B, Cell growth assay. C, S phase cells assay using BrdU. D, Colony formation assay. a, Cell colony picture. b, Cell colony formation rate. Each value was presented as mean ± standard error of the mean (SEM) (Student's t test). Bar ± SEM. **P < .01; *P < .05. E, Cell sphere formation ability. Each value was presented as mean ± standard error of the mean (SEM) (Student's t test). Bar ± SEM. **P < .01; *P < .05