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. 2020 Jan 19;24(5):2942–2955. doi: 10.1111/jcmm.14910

Figure 4.

Figure 4

Importance of LASP1 and CXCR4 for migratory potential. (A) Schematic of a migration chamber and assay conditions: Fluorescent signal from actively migrating cells was detected from below without shine‐through artefacts. Relative migration towards gravitation (B), 10% FCS (C) and 12.5 nmol/L (100 ng/mL) CXCL12 (D). In the presence of CXCR4, knockout of LASP1 resulted in reduced migration. (E) Cytospin and Pappenheim staining of migrated cells. Cytospins were prepared as previously described.9 Results represent the mean of three independent experiments in duplicates ± SD. BSA, bovine serum albumin; CXCL12, C‐X‐C motif chemokine 12; CXCR4, chemokine receptor 4; FCS, foetal calf serum; LASP1, LIM and SH3 domain protein 1; SD, standard deviation