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Gelatin Methacryloyl synthesis and characterization. (A) Gelatin Methacryloyl (GelMA) synthesis by methacrylation at 50 °C produces GelMA and methacrylic acid. (B) Proton nuclear magnetic resonance (1H-MNR) spectra of Gelatin and GelMA, confirming the substitution of primary amine groups by methacryloyl groups in GelMA. (C) Degree of GelMA functionalization, as measured by 2,4,6-Trinitrobenzene Sulfonic Acid (TNBS) assay.
