Figure 3.

Characterization of p47phox siRNA-loaded poly (D,L-lactic-co-glycolic acid) (PLGA) nanoparticles (NPs). siRNA p47phox-encapsulated PLGA nanoparticles were dissolved in water and measured in terms of (A,C) size and (B,D) zeta potential by using a Zetasizer ZS90. (E) Suspended NPs were also assessed by using scanning electron microscopy; scale bar = 200 nm. (F) After 3 days, normal rat knees received intra-articular injections of AAV-mCherry expression vector-loaded PLGA NPs, and they were then were examined under a fluorescent microscope to assess uptake; scale bar = 50 µm. (G) In vitro cumulative siRNA release of PLGA NPs over 48 h. (H) Experimental schematic of the present study that used an MIA-induced OA animal model.