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. 2020 Feb 11;12(2):408. doi: 10.3390/polym12020408

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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(A–C) The FITC labled PR:CCA-NPs were added to B16 cells and co-incubated for 4h to detect the effects of PR: CCA-NPs on pinocytosis by cells, the scale bar represents 25 μm. (A) Control group, (B) DiD labeled cell membrane, (C) FITC labeled PR:CCA-NPs; (D) Effects of different NPs with different concentration on cellular melanin content in B16 cells; E: TEM of PR: CCA5-NPs. Note: * p < 0.05: CCA-NPs (50 μg/mL) vs Free PR; ^※ p < 0.05: CCA1 (50 μg/mL) vs CCA3 (50 μg/mL); ° P < 0.05:CCA1 (50 μg/mL) vs CCA1 (20 μg/mL).