Table 1. Primer sequences for analysis of mRNA gene expression.
mRNA | Primer sequences (5’-3’) | Product size (bp) | GenBank accession number |
---|---|---|---|
GAPDH | F: 5’-GTCGGTTGTGGATCTGACCT-3’ | 207 | NM_001206359.1 |
R: 5’-TTGACGAAGTGGTCGTTGAG-3’ | |||
PCNA | F: 5′-CCTGTGCAAAAGATGGAGTG-3′ | 187 | XM_003359883 |
R: 5′-TTTTCGGTGAGGTGAGAGAGG-3′ | |||
POU5F1 | F: 5′-GCGGACAAGTATCGAGAACC-3′ | 200 | NM_001113060 |
R: 5′-CGTTGCTCTCCTAAAACTCC-3′ | |||
BCL2 | F: 5’-AATGACCACCTAGAGCCTTG-3’ | 182 | NM_214285 |
R: 5’-GGTCATTTCCGACTGAAGAG-3’ | |||
BAX | F: 5’-TGCCTCAGGATGCATCTACC-3’ | 199 | XM_003127290 |
R: 5’-AAGTAGAAAAGCGCGACCAC-3’ | |||
CSF3 | F: 5’- GAGCTTCCTGGAGCTGGCGTAC-3’ | 208 | NM213842 |
R: 5’-TGCTACAGGCGGGAGAAT-3 | |||
CSF3R | F: 5’-CTGGGCCTGCTTCTTGATAA-3’ | 202 | XM_021095950.1 |
R: 5’-GGCTAGTGGACAGGTCTGGA-3 |
F: Forward, R: Reverse.