Table 2. Effects of hrG-CSF supplementation at various concentrations on porcine in vitro development of parthenotes.
hrG-CSF Concentration (ng/mL) | No. of oocytes Cultured (4)* | No. (%) of embryos developed to | Blastocyst cell Number (N) | |
---|---|---|---|---|
Cleaved (%)† | Blastocyst (%)† | |||
0 | 144 | 127 (88.5) | 46 (31.8) | 44.0 ± 1.1(26)a |
10 | 142 | 126 (88.4) | 50 (35.0) | 48.1 ± 1.0(31)bc |
50 | 158 | 143 (90.2) | 45 (28.2) | 49.2 ± 1.0(25)c |
100 | 141 | 125 (88.8) | 46 (32.5) | 44.9 ± 1.2(24)ab |
Values with different superscripts within the same column are significantly different (P < 0.05).
The data represent means ± SEM.
* Number of replicates.
N Number of blastocysts examined.
† Percentage of total cultured oocytes.