Table 3. Effects of hrG-CSF supplementation at various concentrations on porcine pre-implantation embryonic development of in vitro fertilization embryos.
hrG-CSF Concentration (ng/mL) | No. of oocytes Cultured (3) * | No. (%) of embryos developed to | Blastocyst cell Number (N) | |
---|---|---|---|---|
Cleaved (%) † | Blastocyst (%) † | |||
0 | 88 | 62(70.5) | 17(19.3) | 44.3 ± 3.0(17)ab |
10 | 87 | 57(65.5) | 25(28.7) | 62.5 ±4.5 (23)c |
50 | 91 | 58(63.7) | 21(23.1) | 58.4 ± 6.9(21)bc |
100 | 83 | 52(62.7) | 14(16.9) | 41.0 ± 2.9(14)a |
Values with different superscripts within the same column are significantly different (P < 0.05).
The data represent means ± SEM.
* Number of replicates.
N Number of blastocysts examined.
† Percentage of total cultured oocytes.