Table 4. Effects of hrG-CSF (10 ng/mL) treatment duration on in vitro development of parthenotes.
Groups | No. of oocytes | No. (%) of embryos developed to | Blastocyst cell Number (N) | |
---|---|---|---|---|
cultured (3)* | ≥ 2-cells (%)† | Blastocysts (%) † | ||
control | 89 | 66(74.1) | 29(32.8) | 42.0 ± 1.5(28)a |
Day 0 to 3 | 89 | 66(74.2) | 30(33.9) | 45.3 ± 1.7(29)a |
Day 4 to 7 | 89 | 69(77.6) | 28(31.4) | 46.4 ± 1.9(28)a,b |
Day 0 to 7 | 89 | 65(73.2) | 31(34.9) | 51.2 ± 2.1(31)b |
Values with different superscripts within the same column are significantly different (P < 0.05).
The data represent means ± SEM.
hrG-CSF: human recombinant granulocyte-colony stimulating factor.
* Number of replicates.
N Number of blastocysts examined.
† Percentage of total cultured oocytes.