Fig 4. Heatmap of cytokine fold changes from baseline following intrarectal SIVsab infection of AGMs.

Colors represent the fold change from preinfection to postinfection of the cytokines for each of the AGMs. Red indicates a positive fold change, while blue represents a negative fold change, with color intensity being proportional to the magnitude of the fold change, as shown below the heatmap. Animal numbers are shown above the heatmap along with dpi, with the colors below the numbers highlighting their time groups: orange (baseline), green (preramp), blue (ramp-up), red (peak), yellow (set-point), purple (AGM historical controls, late chronic). Cytokines and chemokines are listed on the right side of the heatmap. The results are clustered using a Spearman correlation, with the dendrogram showing relationship displayed on the left. The fold changes for the cytokines and chemokine levels were normalized with a log₂ transformation. The heatmap was generated using the publicly available Morpheus software (Broad Institute).