Skip to main content
. 2020 Mar 2;16(3):e1008333. doi: 10.1371/journal.ppat.1008333

Fig 7. Immunohistochemistry (IHC) for neutrophil infiltration and activation in SIVsab-infected AGMs.

Fig 7

DAB-based IHC for MPO in the (A) transverse colon, and (B) axillary LN of AGMs and chronically SIV-infected RMs. In all the images, positive DAB signal is shown in brown, with the remaining tissue counterstained blue. The representative images of the gut display lateral cuts of the crypts to demonstrate the presence of neutrophils in the surrounding lamina propria. Below are shown image quantifications (C) of the percent area of the total positive DAB signal. The quantification for each animal represents the average of the values from 9–12 individual image quantifications. The four different time groups are based on the days postinfection, with: BL (baseline, preinfection, orange), PRU (preramp, 1–3 dpi, green) RU (ramp-up, 4–6 dpi, blue), PEAK (peak, 9-12dpi, red) and SP (set-point, 46–55 dpi, yellow). The chronic RMs are shown in purple. All AGM quantifications were performed using FIJI version 1.0. Asterisks indicate statistical significance, with * = p< 0.05 and *** = p< 0.001. All AGM images were captured at 200X magnification using an AxioImager M1 bright-field microscope equipped with an AxioCam MRc5. Scale bar: 100 μm.