Figure 6. MyD88 and Toll-2 are expressed in progenitor cells in the adult brain.
(A–D, K) FUCCI expressed with tubGAL80ts, MyD88GAL4 revealed cycling cells in the adult brain. Arrows point to GFP— RFP+ cells in S-phase, GFP+RFP— cells in G1, and GFP+RFP+ cells in G2/M. (D) Quantification: histograms showing that Toll-2 over-expression increased the number of cells in G1, S- and G2/M phases of the cell cycle. (E,F) Some Toll-2pTV>hisYFP+ cells in optic lobes (E) and central brain (F) lack pan-neuronal Elav and pan-glial Repo markers (arrows), meaning they are neither neurons nor glia. (G) Some MyD88 >hisYFP+ cells lack both Repo and Elav markers (arrows), meaning they are neither neurons nor glia. Some but not all large MyD88 >hisYFP+ cells in optic lobes are Repo+. (H,I) Co-localisation of MyD88 >hisYFP with the neuroblast marker anti-Dpn, in central brain and optic lobes (arrowheads). Notice the large MyD88+ cells in optic lobes (H,I), many of which are Dpn+ (I, arrowheads). (H,J) Co-localisation of MyD88 >hisYFP with the neuroblast marker anti-Mira in central brain (arrowheads). Mira also seems to label neurons in adult brains. (K) MyD88+ progenitor cells cycle in the adult brain. Co-localisation of tubGAL80ts, MyD88 >FUCCI with anti-Dpn in adult brains over-expressing Toll-2. Some Dpn+ cells over-expressing Toll-2 were GFP+RFP+ meaning they were in G2/M, and most were GFP+RFP— meaning they were in G1, either just exited mitosis or quiescent. For genotypes, sample sizes and statistical details, see Supplementary file 2. ****p<0.0001. Scale bars: A-G, - I,K : 25 μm; H, J, : 50 μm.