Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (M. musculus) | Lgr6-EGFP-IRES-CreERT2 | Snippert et al., 2010 | Jackson Labs stock no: 016934 RRID:IMSR_JAX:016934 |
Received from H Clevers |
| Genetic reagent (M. musculus) | Gli1-LacZ | Bai et al., 2002 | Jackson Labs stock no: 008211 RRID:IMSR_JAX:008211 |
Received from F Aberger |
| Genetic reagent (M. musculus) | Gli1-CreERT2 | Ahn and Joyner, 2004 | Jackson Labs stock no: 007913 RRID:IMSR_JAX:007913 |
Received from F Aberger |
| Genetic reagent (M. musculus) | Col1a2-CreER | Zheng et al., 2002 | Jackson Labs stock no: 029567 RRID:IMSR_JAX:029567 |
Received from B Crombrugghe |
| Genetic reagent (M. musculus) | R26-tdTomato | Madisen et al., 2010 | Jackson Labs stock no: 007908 RRID:IMSR_JAX:007908 |
Obtained from Jackson Laboratory |
| Genetic reagent (M. musculus) | Ptch1neo(fl)Ex2(fl) | Kasper et al., 2011 | Received from S. Teglund | |
| Genetic reagent (M. musculus) | C57BL/6J | Jackson Labs stock no: 000664 RRID:IMSR_JAX:000664 |
Received from Preclinical Laboratory Karolinska Institutet | |
| Antibody | Rabbit polyclonal anti-K5 | BioLegend | Cat# PRB-160P RRID:AB_291581 |
(1:1000) |
| Antibody | Guinea pig polyclonal anti-K5 | US Biological | Cat# C9097-37 RRID:AB_2134285 |
(1:50) (1:200 for RNA-FISH) |
| Antibody | Rat monoclonal anti-SDC1 | BD Biosciences | Cat# 553712 RRID: AB_394998 |
(1:500) |
| Antibody | Rat monoclonal anti-K8 | DSHB (Developmental Studies Hybridoma Bank) | Cat# TROMA-I RRID:AB_531826 |
(1:1000) |
| Antibody | Rabbit polyclonal anti-K6 | BioLegend | Cat# PRB-169P RRID:AB_10063923 |
(1:2000) |
| Antibody | Guinea pig polyclonal anti-K71 | Progen | Cat# GP-K6irs1 RRID:AB_2716781 |
(1:100) |
| Antibody | Rabbit polyclonal anti-RFP | Rockland | Cat# 600-401-379 RRID:AB_2209751 |
(1:100) |
| Antibody | Rat monoclonal anti-BrdU | Serotec | Cat# OBT0030G RRID: AB_609567 |
(1:400) |
| Antibody | Rabbit polyclonal anti-GFP | Thermo Fisher Scientific | Cat# A-11122 RRID:AB_221569 |
(1:500) |
| Antibody | Rabbit polyclonal anti-Ki67 | Abcam | Cat# ab15580 RRID:AB_443209 |
(1:200) |
| Antibody | Rat monoclonal anti-CD140b | BioLegend | Cat# 136008 RRID:AB_2268091 |
(1:100) |
| Chemical compound, drug | TO-PRO-3 | Invitrogen | Cat# T3605 | (1:1000) |
| Chemical compound, drug | Hoechst 33342 | Invitrogen | Cat# H3570 | (1 µg/mL) |
| Chemical compound, drug | DAPI | Invitrogen | Cat# D1306 |
(1 µg/mL) |
| Chemical compound, drug | Tamoxifen | Sigma | Cat# T5648 | |
| Chemical compound, drug | 4-OH Tamoxifen | Sigma | Cat# H6278 | |
| Chemical compound, drug | NBT/BCIP stock solution | Roche | Cat# 11681451001 | |
| Chemical compound, drug | Vismodegib, Free Base | LC Laboratories | Cat# V-4050 | |
| Commercial assay or kit | RNAscope Multiplex Fluorescent Kit v2 | ACDBio/Bio-Techne | Cat# 323100 | |
| Commercial assay or kit | TSA Cy 3, Cy 5, TMR, Fluorescein Evaluation Kit | Perkin Elmer | Cat# NEL760001KT | |
| Sequence-based reagent | 3-plex Positive Control Probe | ACDBio/Bio-Techne | Cat# 320881 | RNA-FISH Probe |
| Sequence-based reagent | 3-plex Negative Control Probe | ACDBio/Bio-Techne | Cat# 320871 | RNA-FISH Probe |
| Sequence-based reagent | Mm-Gli1 | ACDBio/Bio-Techne | Cat# 311001 | RNA-FISH Probe |