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. Author manuscript; available in PMC: 2021 Apr 1.
Published in final edited form as: Acta Physiol (Oxf). 2020 Jan 8;228(4):e13433. doi: 10.1111/apha.13433

Figure 1. Transplantation setup.

Figure 1.

(A) Experimental design (B) Confocal imaging of control and diphtheria toxin (DT)-ablated islet sections (insulin –red, glucagon –green, scale bar: 70μm), (C) Efficiency of DT-induced β-cell ablation in RIP-DTR NSG strain expressed as % insulin cells / islet section. (D) Glycemia and (E) Weight evolution in ablated and control mice (chartreuse – 1w-posTX, blue – 4w-postTX, TX – transplant, DT – DT-induced ablation), (F) Binocular imaging of the mounted alginate capsules and (G) 3D reconstructions of the immunofluorescence on whole alginate beads of the 4 conditions analyzed of the 4 conditions analyzed following the retrieval procedure (insulin – green, and DAPI – blue, scale bar: 200μm) (H) High magnification confocal imaging of insulin+ cells (scale bar: 5μm). Data are shown as mean ± SD.