Table 1:

Summary of methods used to generate adipocytes from hiPSCs

Study	Technique	Mesoderm induction	Mesoderm markers	MSC phenotype	Adipocyte	Adipogenesis induction
Taura et al.73	EB outgrowth	ES cell culture medium w/out FGF2	Unknown	Unknown	Unknown	INS, IBMX, Dex, Indo, pioglitazone
Ahfeldt et al.37	EB outgrowth, transgene	DMEM, 15% FBS	GSC/TBX3	CD73, CD105	White	INS, Dex, Rosi, PPARG transgene
Ahfeldt et al.37	EB outgrowth, transgene	DMEM, 15% FBS	GSC/TBX3	CD73, CD105	Brown	INS, Dex, Rosi, PPARG + CEBPB transgenes
Nishio et al.76	EB outgrowth	BPEL medium, BMP4, VEGFA, KITLG FLT3LG, IL-6, IGF-II	MYF5, PDFGRα, PAX7, PAX3	Unknown	Brown	BMP7, VEGFA, KITLG FLT3LG, IL-6, IGF-2
Mohsen-Kanson, et al.77	EB outgrowth	DMEM/F12/20% KSR + retinoic acid	Unknown	CD73, CD105, CD90, CD146, PAX3	White	INS, IBMX, Dex, T3, Rosi
Mohsen-Kanson, et al.77	EB outgrowth	DMEM/F12/20% KSR	PAX3?	CD73, CD105, CD90, CD146, PAX3	Brown	INS, IBMX, Dex, T3, Rosi
Hafner et al.80	EB outgrowth	DMEM/F12/20% KSR	PAX3?	CD73, CD105, CD90	Brown and Beige	INS, IBMX, Dex, T3, Rosi, SB431542, ascorbic acid, hydrocortisone, EGF
Guénantin et al.84	Monolayer	STEMPro34 HSC medium + BMP4, Activin A	TBOX, MESP1	PDGFRα, CD44, CD29 and LY6E	Beige	INS, IBMX, Dexa, Indo
Su et al.80	Monolayer	STEMdiff™ Mesoderm Induction Medium	FOXF1, BAPX1, HOX11	CD73, CD105, CD90, CD146, PDGFRβ, NG2	Beige	INS, IBMX, Dex, Indo, T3, Rosi, SB431542, ascorbic acid, hydrocortisone, EGF

EB – embryoid body, INS – insulin, Dex – dexamethasone, Indo – indomethacin, Rosi – Rosiglitazone, BPEL - Bovine Serum Albumin (BSA) Polyvinylalchohol Essential Lipids Medium, HSC – hematopoietic stem cell.