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. 2020 Feb 13;20:205–216. doi: 10.1016/j.omtn.2020.02.007

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© 2020 The Author(s)

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Overexpressed miR-17-5p and Inhibited PVT1 Decelerate Apoptosis of Ovarian Granulosa Cells in PCOS

(A) Cell cycle distribution of ovarian granulosa cells in each group was detected by flow cytometry. (B) Cartogram of cell cycle distribution of ovarian granulosa cells in each group. (C) Apoptosis of ovarian granulosa cells in each group was detected by flow cytometry. (D) Cartogram of apoptosis of ovarian granulosa cells in each group. (E) Representative images of Hoechst 33258 staining. (F) Apoptosis of ovarian granulosa cells was measured by TUNEL staining. (G) Statistical results of TUNEL staining. (H) Protein expression of cleaved-caspase-3, Bax, and Bcl-2 of ovarian granulosa cells in each group. The data were all measurement data and are expressed as mean ± standard deviation. ^ap < 0.05 versus the sh-NC group, ^bp < 0.05 versus the sh-PVT1 group, ^cp < 0.05 versus the mimic NC group, ^dp < 0.05 versus the oe-NC group, ^ep < 0.05 versus the oe-PVT1 group.