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. 2020 Feb 11;20:242–251. doi: 10.1016/j.omtn.2020.02.001

Figure 6.

Figure 6

OPN Upregulates Col IV Expression in HRCECs

(A) The expressions of OPN in retinas of C57 and STZ-induced diabetic mice were examined by quantitative real-time PCR. (B and C) mRNA and protein levels of Col IV in HRCECs after exogenous rhOPN (400 ng/mL) treatment for 48 h. (D–F) HRCECs were transfected with SPP1-OPN, as well as respective scrambled control for 72 h. mRNA and protein levels of OPN and Col IV were determined by quantitative real-time PCR and western blotting analysis. (G–I) HRCECs were transfected with OPN-siRNA, as well as respective scrambled control for 72 h. mRNA and protein levels of OPN and Col IV were determined by quantitative real-time PCR and western blotting analysis. Data were presented as mean ± SD. N = 3 per group; **p < 0.01 and ***p < 0.001.