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. 2020 Jan 29;45(4):809–824. doi: 10.1007/s11064-020-02954-y

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© The Author(s) 2020

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Fig. 2 — Liberation of copper ions from CuO-NPs and CuO-Fe-NPs in the presence of the copper chelator BCS. CuO-NPs, CuO-Fe-NPs or CuCl₂ in concentrations of 100 µM in IB-BSA were mixed with 1 mM BCS (in IB-BSA) in the absence (a) or the presence of 1 mM ascorbate (b) and the increase in absorbance of the Cu⁺-BCS complex at a wavelength of 484 nm was monitored for up to 30 min (a, b) or recorded after 24 h of incubation (c). The data shown represent means ± SD of values obtained in three independent experiments